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| Registros recuperados: 38 | |
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| Hadjadji, Imene; Frehi, Hocine; Ayada, Lembarek; Abadie, Eric; Collos, Yves. |
| Environmental conditions ultimately leading to blooms of the toxic dinoflagellate Alexandrium catenella/tamarense were investigated at two Mediterranean sites (Annaba Bay, Algeria and Thau lagoon, France). Three years were examined in details: 1992 (a pre-Alexandrium period), 2002 (a year with the first bloom in Annaba) and 2010 (a year with a major bloom in Annaba). Most conditions were similar, but ammonium concentrations were much higher in Annaba (up to 100 μM) than in Thau (up to 10 μM). First records of A. catenella/tamarense were in 1995 for Thau and 2002 for Annaba, and coincided with soluble reactive phosphorus (SRP) decreasing below a concentration of about 1 μM. No other environmental variable could be related to those blooms. Thus, it is likely... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella/tamarense; Blooms; Algeria; France; Mediterranean. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00174/28574/26995.pdf |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher. |
| beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral... |
| Tipo: Text |
Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf |
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| Abadie, Eric; Zouher, Amzil; Belin, Catherine; Comps, Marie-annick; Elziere-papayanni, Panayota; Lassus, Patrick; Le Bec, Claude; Marcaillou-le Baut, Claire; Nezan, Elizabeth; Poggi, Robert. |
| A bloom of the toxic dinoflagellate Alexandrium tamarense was observed for the first time in Thau iagoon between November and December 1998. Maximum cell concentrations detected in shellfish farming areas (85 000 Cells/1) were high enough to make mussels and clams unsuitable for human consumption (max. 850 ug. STX eq.100 g'1) while toxic levels in oysters did not exceed, or even reach, the international public health threshold established for PSP toxins. This event, new to French Mediterranean waters, was investigated by both local and national veterinary services and by the Ifremer teams involved in the National Monitoring Network for Phytoplankton and Phycotoxines (Réphy). The chronological and operational description of the event was compared with data... |
| Tipo: Text |
Palavras-chave: Alexandrium; Monitoring; Toxic dinoflagellates; Thau lagoon; Alexandrium; Surveillance; Dinoflagellés toxiques; Etang de Thau. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-884.pdf |
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| Miossec, Laurence; Besseau, Laetitia; Haugarreau, Larissa; Le Guyader, Francoise; Menard, Dominique; Pommepuy, Monique; Abadie, Eric; Comps, Marie-annick; Guillou, Jean-louis; Le Bec, Claude; Sauvagnargues, Jean-claude; Bon, Fabienne; Jamot, B.; Kohli E, Evelyne; Potier, P.; Daurat, G.; Guyonnet, J.p.. |
| Les microorganismes, bactéries et virus d'origine entérique, sont abondants dans les rejets urbains. Si la charge bactériologique contenue dans les eaux usées est notablement réduite lors des traitements appliqués dans les stations d'épurations, de nombreux microorganismes persistent et sont rejetés avec les eaux épurées dans le milieu récepteur, en rivière ou directement en mer. Le développement de méthodes analytiques basées sur la biologie moléculaire a permis récemment la détection de virus entériques non cultivables dans les échantillons de l'environnement, tels que les entérovirus, le virus de l'hépatite A, les calicivirus, les astrovirus et les rotavirus. En France, les données publiées sur la présence de ces virus entériques, détectés par RTPCR,... |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00044/15552/12941.pdf |
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| Collos, Y; Vaquer, A; Laabir, M; Abadie, Eric; Laugier, Thierry; Pastoureaud, Annie; Souchu, Philippe. |
| A monitoring program with a weekly sampling frequency over a 15-month period indicates that urea concentrations above a certain threshold level may trigger the blooms of Alexandrium catenella in Thau lagoon. However, urea concentrations were also sometimes related to ammonium and dissolved organic nitrogen concentrations, indicating that the role of urea may not be a direct one. An original approach is used to assess the relative contribution of several nitrogen sources (nitrate, nitrite, ammonium, urea) to growth of A. catenella by comparing nitrogen uptake rates to nitrogen-based growth rates estimated from dilution experiments during four blooms over a 4-year period (2001-2004) in Thau lagoon. Nitrate and nitrite contributed 0.1-14% and 0.1-5%... |
| Tipo: Text |
Palavras-chave: Uptake kinetics; Ammonium; Urea; Growth; Nitrogen; HAB; Alexandrium catenella. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-6454.pdf |
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| Masseret, Estelle; Banack, Sandra; Boumediene, Farid; Abadie, Eric; Brient, Luc; Pernet, Fabrice; Juntas-morales, Raoul; Pageot, Nicolas; Metcalf, James; Cox, Paul; Camu, William. |
| Background Dietary exposure to the cyanotoxin BMAA is suspected to be the cause of amyotrophic lateral sclerosis in the Western Pacific Islands. In Europe and North America, this toxin has been identified in the marine environment of amyotrophic lateral sclerosis clusters but, to date, only few dietary exposures have been described. Objectives We aimed at identifying cluster(s) of amyotrophic lateral sclerosis in the Hérault district, a coastal district from Southern France, and to search, in the identified area(s), for the existence of a potential dietary source of BMAA. Methods A spatio-temporal cluster analysis was performed in the district, considering all incident amyotrophic lateral sclerosis cases identified from 1994 to 2009 by our expert... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00168/27941/26234.pdf |
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| Abadie, Eric; Kaci, Lamia; Berteaux, Tom; Hess, Philipp; Sechet, Veronique; Masseret, Estelle; Rolland, Jean-luc; Laabir, Mohamed. |
| ulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s−1. Results showed that ammonium was assimilated by cells more rapidly... |
| Tipo: Text |
Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Ingril lagoon; Growth; Nitrogen source. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00276/38748/37290.pdf |
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| Abadie, Eric. |
| Harmful and / or toxic phytoplankton blooms impact for a long time marine ecosystems worldwide. These massive developments have an adverse effect on ecosystems and their exploitation. The lagoons of the region Languedoc-Roussillon are affected for decades. In addition to recurring health risks from Dinophysis (DSP toxins) and Alexandrium (PSP toxins), Vulcanodinium rugosum was identified in 2011 in the lagoon Ingril. This new species product pinnatoxins (neurotoxins). Through this thesis work, the biology of this new species (life cycle, growth condition, toxin production), its shellfish contamination capacity and geographic distribution in neighboring lagoons were studied on cultures in the laboratory and via environmental monitoring. The results of this... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00473/58425/61002.pdf |
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| Marzin, Anahita; Crottier, Anais; Piquet, Jean-come; Abadie, Eric; Roque D'Orbcastel, Emmanuelle; Fiandrino, Annie. |
| L’étude de zone des lotissements conchylicoles de la lagune de Thau (34.39) a été menée, à la demande du Comité Régional de la Conchyliculture en Méditerranée et du préfet de l’Hérault, pour étudier la possibilité de sectoriser la zone de production en sous-zones, au regard du risque microbiologiques (REMI). Une étude de dossier a été réalisée afin de recenser les principales sources de contamination. Celles-ci sont essentiellement d’origine anthropique et impactent différemment les secteurs envisagés. Des prélèvements de coquillages du groupe 3 ont été réalisés de mai 2017 à mai 2018 avec une fréquence bimensuelle. Au regard des critères du règlement (CE) n° 854/2004, les résultats des analyses microbiologiques (indicateur Escherichia coli) ont permis... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Étude de zone; Lagune de Thau; Département de l’Hérault. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00459/57056/58965.pdf |
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| Crottier, Anais; Abadie, Eric; Brun, Melanie; Piquet, Jean-come; Fiandrino, Annie. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats des réseaux de contrôle microbiologique (REMI) et de surveillance chimique (ROCCH), ce rapport inclut un bilan national et décrit le programme annuel des départements de l’Hérault, du Gard et de l’Aude. Il présente l’ensemble des résultats obtenus, en particulier l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées pour la période 2015-2017. Sur les 29 zones de production conchylicole classées et suivies en Occitanie, la qualité a pu être estimée pour 23 d’entre elles. Selon les critères réglementaires pris en compte (règlement CE n°854/2004, modifié par le règlement n°2285/2015), dans les départements de... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de l’Hérault; Départemen tdu Gard; Département de l’Aude. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00445/55635/57280.pdf |
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| Abi-khalil, Celina; Lopez-joven, Carmen; Abadie, Eric; Savar, Veronique; Amzil, Zouher; Laabir, Mohamed; Rolland, Jean-luc. |
| The multifactorial etiology of massive Crassostrea gigas summer mortalities results from complex interactions between oysters, opportunistic pathogens and environmental factors. In a field survey conducted in 2014 in the Mediterranean Thau Lagoon (France), we evidenced that the development of the toxic dinoflagellate Alexandrium catenella, which produces paralytic shellfish toxins (PSTs), was concomitant with the accumulation of PSTs in oyster flesh and the occurrence of C. gigas mortalities. In order to investigate the possible role of toxic algae in this complex disease, we experimentally infected C. gigas oyster juveniles with Vibrio tasmaniensis strain LGP32, a strain associated with oyster summer mortalities, after oysters were exposed to Alexandrium... |
| Tipo: Text |
Palavras-chave: Harmful algae; Environment; Interaction; Pathogens; Defense; Paralytic shellfish toxin. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00309/42016/41308.pdf |
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| Zendong, Zita; Herrenknecht, Christine; Abadie, Eric; Brissard, Charline; Tixier, Celine; Mondeguer, Florence; Sechet, Veronique; Amzil, Zouher; Hess, Philipp. |
| Marine biotoxins are algal metabolites that can accumulate in fish or shellfish and render these foodstuffs unfit for human consumption. These toxins, released into seawater during algal occurrences, can be monitored through passive sampling. Acetone, methanol and isopropanol were evaluated for their efficiency in extracting toxins from algal biomass. Isopropanol was chosen for further experiments thanks to a slightly higher recovery and no artifact formation. Comparison of Oasis HLB, Strata-X, BondElut C18 and HP-20 sorbent materials in SPE-mode led to the choice of Oasis HLB, HP-20 and Strata-X. These three sorbents were separately exposed as passive samplers for 24 h to seawater spiked with algal extracts containing known amounts of okadaic acid (OA),... |
| Tipo: Text |
Palavras-chave: Passive sampling; Oasis HLB; Strata-X; LOPE; PDMS; SPATT. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00186/29756/28187.pdf |
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| Arnich, Nathalie; Abadie, Eric; Delcourt, Nicolas; Fessard, Valérie; Fremy, Jean-marc; Hort, Vincent; Lagrange, Emmeline; Maignien, Thomas; Molgó, Jordi; Peyrat, Marie-bénédicte; Vernoux, Jean-paul; Mattei, César. |
| Pinnatoxins (PnTXs) are a group of emerging marine biotoxins produced by the benthic dinoflagellate Vulcanodinium rugosum, currently not regulated in Europe or in any other country in the world. In France, PnTXs were detected for the first time in 2011, in mussels from the Ingril lagoon (South of France, Mediterranean coast). Since then, analyses carried out in mussels from this lagoon have shown high concentrations of PnTXs for several months each year. PnTXs have also been detected, to a lesser extent, in mussels from other Mediterranean lagoons and on the Atlantic and Corsican coasts. In the French data, the main analog is PnTX G (low levels of PnTX A are also present in some samples). No cases of PnTXs poisoning in humans have been reported so far in... |
| Tipo: Text |
Palavras-chave: Pinnatoxins; Shellfish; Emerging marine biotoxins; Risk assessment. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00619/73105/72236.pdf |
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| Laabir, Mohamed; Collos, Yves; Masseret, Estelle; Grzebyk, Daniel; Abadie, Eric; Savar, Veronique; Sibat, Manoella; Amzil, Zouher. |
| Laboratory experiments were designed to study the toxin content and profile of the Alexandrium catenella strain ACT03 (isolated from Thau Lagoon, French Mediterranean) in response to abiotic environmental factors under nutrient-replete conditions. This dinoflagellate can produce various paralytic shellfish toxins with concentrations ranging from 2.9 to 50.3 fmol/cell. The toxin profile was characterized by carbamate toxins (GTX3, GTX4 and GTX5) and N-sulfocarbamoyl toxins (C1, C2, C3 and C4). C2 dominated at 12-18 degrees C, but only for salinities ranging from 10 to 25 psu, whereas GTX5 became dominant at temperatures ranging from 21 to 30 degrees C at almost all salinities. There was no significant variation in the cellular toxin amount from 18 degrees C... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella; PSP toxins; Temperature; Salinity; Light. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25969/24056.pdf |
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| Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle. |
| Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic... |
| Tipo: Text |
Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf |
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| Lassus, Patrick; Amzil, Zouher; Baron, Regis; Sechet, Veronique; Barille, Laurent; Abadie, Eric; Bardouil, Michele; Sibat, Manoella; Truquet, Philippe; Berard, Jean-baptiste; Gueguen, Marielle. |
| In October and November 2003 a bloom of the toxic dinoflagellate Alexandrium catenella was observed in the North-east zone of Thau lagoon (French Mediterranean coast). Sea water samples were collected every hours to evaluate time-related variations of phytoplankton concentrations and to compare the relative ratio of A. catenella versus other phytoplankton species during the outbreak. From these observations, trials using recirculated sea water systems were performed to: i) evaluate the physiological effects on oyster of increasing proportions of A. catenella within a mixed microalgal diet where the non-toxic diatom Thalassiosira weissflogii was present at concentration: 1500 cells ml(-1), ii) compare the effect of two temperatures (12 degrees C and 18... |
| Tipo: Text |
Palavras-chave: Mediterranean Sea; Alexandrium catenella; Toxin content; Model; Crassostrea gigas; Paralytic shellfish poisoning. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2624.pdf |
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| Collos, Yves; Bec, Beatrice; Jauzein, Cecile; Abadie, Eric; Laugier, Thierry; Lautier, Jacques; Pastoureaud, Annie; Souchu, Philippe; Vaquer, Andre. |
| Time series data have been examined in Thau lagoon (Southern France) from 1972 to 2006 for water temperature, salinity, nutrients and from 1987 to 2006 for phytoplankton. A first main trend identified is an increase in mean annual water temperature (1.5 degrees C over 33 years or 0.045 degrees C/year) that was not evenly distributed among seasons. The highest rate of increase was in the spring (+ 3.0 degrees C over 33 years), followed by summer (+ 2.0 degrees C and fall (+ 1.7 degrees C. In winter, no significant increase over the 33 year period could be found. A second clear trend is a large decrease in soluble reactive phosphorus (SRP) concentration over the same 33 year period (summer values decreased from 10 mu M to 1 mu M. while winter values... |
| Tipo: Text |
Palavras-chave: Skeletonema costatum; Alexandrium catenella; Picocyanobacteria; Oligotrophication; Long term variability; Thau lagoon. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6420.pdf |
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| Registros recuperados: 38 | |
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